polymer-based peroxidase staining method Search Results


vectastain elite abc kit  (Vector Laboratories)
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Vector Laboratories vectastain elite abc kit
Vectastain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain elite abc kit - by Bioz Stars, 2026-08
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glutathione peroxidase assay kit  (Cayman Chemical)
90
Cayman Chemical glutathione peroxidase assay kit
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Glutathione Peroxidase Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m6101 vectastain elite abc kit vector laboratories  (Vector Laboratories)
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Vector Laboratories m6101 vectastain elite abc kit vector laboratories
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
M6101 Vectastain Elite Abc Kit Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pi 1000 rrid ab 2336198 horse anti mouse peroxidase  (Vector Laboratories)
96
Vector Laboratories pi 1000 rrid ab 2336198 horse anti mouse peroxidase
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Pi 1000 Rrid Ab 2336198 Horse Anti Mouse Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pi 1000 rrid ab 2336198 horse anti mouse peroxidase - by Bioz Stars, 2026-08
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horseradish peroxidase hrp conjugated goat anti rabbit  (Jackson Immuno)
97
Jackson Immuno horseradish peroxidase hrp conjugated goat anti rabbit
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Horseradish Peroxidase Hrp Conjugated Goat Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase hrp conjugated goat anti rabbit - by Bioz Stars, 2026-08
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peroxidase conjugated goat anti rabbit igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology peroxidase conjugated goat anti rabbit igg
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Peroxidase Conjugated Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2026-08
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sheep anti mouse igg hrp conjugated secondary antibody  (GE Healthcare)
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GE Healthcare sheep anti mouse igg hrp conjugated secondary antibody
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Sheep Anti Mouse Igg Hrp Conjugated Secondary Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sheep anti mouse igg hrp conjugated secondary antibody - by Bioz Stars, 2026-08
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horseradish peroxidase hrp polymer detection system  (Thermo Fisher)
99
Thermo Fisher horseradish peroxidase hrp polymer detection system
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Horseradish Peroxidase Hrp Polymer Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp prdx2 mm00448996 m1  (Thermo Fisher)
85
Thermo Fisher gene exp prdx2 mm00448996 m1
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Gene Exp Prdx2 Mm00448996 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp mpo hs00924296 m1  (Thermo Fisher)
93
Thermo Fisher gene exp mpo hs00924296 m1
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Gene Exp Mpo Hs00924296 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp gpx1 hs00829989 gh  (Thermo Fisher)
98
Thermo Fisher gene exp gpx1 hs00829989 gh
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Gene Exp Gpx1 Hs00829989 Gh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp gpx2 mm00850074 g1  (Thermo Fisher)
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Thermo Fisher gene exp gpx2 mm00850074 g1
To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by <t>Cayman's</t> Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.
Gene Exp Gpx2 Mm00850074 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by Cayman's Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.

Journal: Cancer letters

Article Title: Comparative analysis of the relative potential of silver, zinc-oxide and titanium-dioxide nanoparticles against UVB-induced DNA damage for the prevention of skin carcinogenesis

doi: 10.1016/j.canlet.2016.09.026

Figure Lengend Snippet: To examine the comparative effect of Ag-, ZnO-, and TiO2-NPs pretreatment on the anti-oxidant activity of cells, HaCaT cells (1×106/plate) were seeded in glass plates and treated with NPs (2 μg/mL) for 3 h prior to UVB-irradiation. (A) After 24 h, cells were lysed and the total anti-oxidant capacity was measured by Cayman's Total Anti-Oxidant Capacity Kit as per manufacturer's instructions. (B) To examine the effect of NPs treatment on gene expression level of anti-oxidant enzymes, cells were harvested 24 h after UVB-irradiation and cellular RNA was extracted. The relative mRNA expression level of SOD, CAT and GPx was analyzed by RT-PCR. Bars represent mean ± S.D.; “*” (p<0.05). (C) The enzymatic activity of SOD, CAT and GPx was measured as per instructions by Cayman's superoxide dismutase, catalase and glutathione peroxidase assay kits, respectively.

Article Snippet: GPx activity was measured using Cayman's Glutathione Peroxidase Assay Kit (Cayman Chemical Company) as per manufacturer's instructions.

Techniques: Activity Assay, Irradiation, Expressing, Reverse Transcription Polymerase Chain Reaction, Glutathione Peroxidase Assay